Collagen I alpha 1 Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody

Supplier:  Novus Biologicals NBP229651

Catalog No. NBP229651

Description

Collagen I alpha 1 Polyclonal specifically detects Collagen I alpha 1 in Human, Mouse, Rat samples. It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Collagen I alpha 1
• Classification: Polyclonal
• Dilution: Western Blot 1:1000, Immunohistochemistry 1:100, Immunohistochemistry-Paraffin 1:100
• Gene Accession No.: P02452
• Gene Symbols: COL1A1
• Immunogen: This Collagen I alpha 1 antibody was raised against synthetic peptide corresponding to amino acid residues specific to the collagen 1, alpha 1 propeptide conjugated to KLH. Accession # P02452
• Purification Method: Antigen Affinity-purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This Collagen I alpha 1 antibody is specific for the propeptide portion of the ∼180 kDa collagen I alpha1 polypeptide found in human lung fibroblast extract. The antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 C in 10 mM sodium citrate (pH 6.0)/ 0.1% Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized.
• Content And Storage: Store at -20C. Avoid freeze-thaw cycles.

• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Formulation: PBS with No Preservative
• Gene Alias: Alpha-1 type I collagen, COL-IA1, Collagen 1, Collagen 1 alpha 1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen of skin, tendon and bone, alpha-1 chain, Collagen Type I Alpha 1 Chain, collagen, type I, alpha 1, Collagen1, Collagen-1, EDSARTH1, OI4, pro-alpha-1 collagen type 1, Type I Procollagen Alpha 1 Chain
• Host Species: Rabbit
• Molecular Weight of Antigen: 180 kDa
• Quantity: 0.1 mL
• Research Discipline: Cell Biology, Cellular Markers, Extracellular Matrix, Signal Transduction, Stem Cells
• Gene ID (Entrez): 1277
• Target Species: Human, Mouse, Rat
• Isotype: IgG

For Research Use Only