Collagen II Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 4 publications

Supplier:  Novus Biologicals NBP177795

Catalog No. NBP177795

Description

Collagen II Polyclonal specifically detects Collagen II in Human, Mouse, Rat, Bovine, Sheep samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

• Antigen: Collagen II
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Alias: Alpha-1 type II collagen, ANFH, AOM, cartilage collagen, chondrocalcin, COL11A3, Collagen 2, collagen alpha-1(II) chain, collagen II, alpha-1 polypeptide, collagen type 2, collagen, type II, alpha 1, collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital), Collagen-2, MGC131516, SEDC
• Host Species: Rabbit
• Molecular Weight of Antigen: 141.79 kDa
• Quantity: 0.1 mg
• Research Discipline: Cytoskeleton Markers, Extracellular Matrix, Stem Cell Markers
• Gene ID (Entrez): 1280
• Target Species: Human, Mouse, Rat, Bovine, Sheep, Canine, Silkworm
• Isotype: IgG

• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Concentration: 1 mg/ml
• Dilution: Western Blot 1:1000-1:10000, ELISA 1:5000-1:50000, Immunohistochemistry 1:50-1:400, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen
• Gene Accession No.: P02458
• Gene Symbols: COL2A1
• Immunogen: Collagen II from human knee cartilage and bovine nasal cartilage. (Uniprot: P02458)
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen II and has expected cross-reactivity with Type IV and VI and negligible cross reactivity to Type I, III and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
• Content And Storage: Store at 4°C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

For Research Use Only