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Collagen III alpha 1/COL3A1 Antibody, Novus Biologicals™
SDP

Catalog No. NB600594SS Shop All R&D Systems Products
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NB600594 0.1 mg
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Rabbit Polyclonal Antibody has been used in 21 publication

Collagen III alpha 1/COL3A1 Polyclonal specifically detects Collagen III alpha 1/COL3A1 in Human, Mouse, Rat, Bovine, Feline samples. It is validated for Western Blot, Simple Western, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin.

Specifications

Antigen Collagen III alpha 1/COL3A1
Applications Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Classification Polyclonal
Concentration 1 mg/ml
Conjugate Unconjugated
Dilution Western Blot 1:1000-1:10000, Simple Western, Flow Cytometry, ELISA 1:5000-1:50000, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:50 - 1:200
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
Gene Accession No. P02461
Gene Alias alpha1 (III) collagen, Collagen 3, collagen alpha-1(III) chain, collagen, fetal, collagen, type III, alpha 1, Collagen-3, EDS4A, Ehlers-Danlos syndrome type IV, autosomal dominant, FLJ34534
Gene Symbols COL3A1
Host Species Rabbit
Immunogen Collagen III alpha 1/COL3A1 from human and bovine placenta (Uniprot: P02461)
Molecular Weight of Antigen 139 kDa
Purification Method Affinity Purified
Quantity 0.02 mg
Regulatory Status RUO
Research Discipline Cancer, Cell Biology, Extracellular Matrix
Primary or Secondary Primary
Gene ID (Entrez) 1281
Test Specificity Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen III alpha 1/COL3A1 and has expected cross-reactivity with Type I and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
Target Species Human, Mouse, Rat, Bovine, Feline
Content And Storage Store at 4°C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Isotype IgG
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