Collagen IV Antibody, Novus Biologicals™

Catalog No.	Quantity
NB1206586	0.1 mg
NB1206586SS	0.02 mg

Catalog No. NB1206586 Supplier Novus Biologicals Supplier No. NB1206586

Description

Rabbit Polyclonal Antibody

Collagen IV Polyclonal specifically detects Collagen IV in Human, Mouse, Rat, Bovine, Feline samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Knockout Validated, Super-Resolution Microscopy.

Specifications

• Antigen: Collagen IV alpha 1
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunohistochemistry (Free Floating), Immunoassay, CyTOF
• Classification: Polyclonal
• Concentration: 1 mg/ml
• Conjugate: Unconjugated
• Dilution: Western Blot 1:1000-1:10000, ELISA 1:5000-1:50000, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:50-1:200, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Knockout Validated, Super-Resolution Microscopy
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Accession No.: P02462
• Gene Alias: alpha-1 chain, arresten, COL4A1 NC1 domain, collagen alpha-1(IV) chain, collagen IV, alpha-1 polypeptide, collagen, type IV, alpha 1, Collagen-4, EC 3.4.23, EC 6.3.1.2
• Gene Symbols: COL4A1
• Host Species: Rabbit
• Immunogen: Collagen IV from human and bovine placenta (Uniprot: P02462)
• Molecular Weight of Antigen: 160.61 kDa
• Purification Method: Affinity Purified
• Quantity: 0.1 mg
• Regulatory Status: RUO
• Research Discipline: Cytoskeleton Markers, Extracellular Matrix, Stem Cell Markers
• Primary or Secondary: Primary
• Gene ID (Entrez): 1282
• Test Specificity: Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen IV and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
• Target Species: Human, Mouse, Rat, Bovine, Feline, Mammalia
• Content And Storage: Store at 4°C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
• Isotype: IgG

For Research Use Only