Collagen IV Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody

Supplier:  Novus Biologicals NB1206586

Catalog No. NB1206586

Description

Collagen IV Polyclonal specifically detects Collagen IV in Human, Mouse, Rat, Bovine, Feline samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Knockout Validated, Super-Resolution Microscopy.

Specifications

• Antigen: Collagen IV alpha 1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Alias: alpha-1 chain, arresten, COL4A1 NC1 domain, collagen alpha-1(IV) chain, collagen IV, alpha-1 polypeptide, collagen, type IV, alpha 1, Collagen-4, EC 3.4.23, EC 6.3.1.2
• Host Species: Rabbit
• Molecular Weight of Antigen: 160.61 kDa
• Quantity: 0.1 mg
• Research Discipline: Cytoskeleton Markers, Extracellular Matrix, Stem Cell Markers
• Gene ID (Entrez): 1282
• Target Species: Human, Mouse, Rat, Bovine, Feline, Mammalia
• Isotype: IgG

• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunohistochemistry (Free Floating), Immunoassay, CyTOF
• Concentration: 1 mg/ml
• Dilution: Western Blot 1:1000-1:10000, ELISA 1:5000-1:50000, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:50-1:200, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Knockout Validated, Super-Resolution Microscopy
• Gene Accession No.: P02462
• Gene Symbols: COL4A1
• Immunogen: Collagen IV from human and bovine placenta (Uniprot: P02462)
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen IV and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
• Content And Storage: Store at 4°C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

For Research Use Only