Collagen X Monoclonal Antibody (X53), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-2853	Invitrogen™ 14977180	25 μg
50-112-2854	Invitrogen™ 14977182	100 μg

Description

Description: The monoclonal antibody X53 recognizes human, mouse, porcine, guinea pig, and rat Collagen Type X. X53 does not cross-react with collagen types I, II, III, IV, or VI. Collagen Type X is a homotrimeric short chain collagen. Collagen Type X has limited expression in normal adult tissues, primarily being expressed during bone growth and development. Expression is localized to the peri- and extracellular matrix of hypertrophic chondrocytes during endochondral ossification and also plays a role in cartilage mineralization. Collagen Type X is elevated in many tumor types with expression being localized in the vasculature. Mutations to the gene COL10A1, which encodes human Collagen Type X, is associated with Schmid type metaphyseal chondrodysplasia. Applications Reported: This X53 antibody has been reported for use in microscopy, immunohistochemical staining, ELISA, and immunocytochemistry. Applications Tested: This X53 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.

Specifications

• Antigen: Collagen X
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: Col10; COL10A1; Col10a-1; Collagen alpha-1(X) chain; collagen type X alpha 1 chain; collagen X, alpha-1 polypeptide; collagen, type X, alpha 1; collagen, type X, alpha 1(Schmid metaphyseal chondrodysplasia); pro alpha 1; procollagen, type X, alpha 1; Schmid metaphyseal chondrodysplasia; type X collagen; type X collagen, alpha 1
• Gene Symbols: COL10A1
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Gene: COL10A1

• Clone: X53
• Applications: ELISA, Immunohistochemistry (Paraffin), Immunocytochemistry
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human, Mouse, Rat, Canine, Pig, Guinea Pig
• Gene Accession No.: Q03692, Q05306
• Gene ID (Entrez): 100856711, 106026924, 12813, 1300, 25681, 448809
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.