Novus Biologicals™ Complement Component C5/C5a Antibody Pair [HRP]

Mouse Monoclonal Antibody Pair

Supplier:  Novus Biologicals™ NBP27967615PLATES

Catalog No. NB010391

Description

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Complement Component C5/C5a.

Specifications

• Assay Range: 23.44 pg/mL
• Assay: Sandwich ELISA
• Immunoassay Kit Format: Sandwich ELISA Kit
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Complement Component C5/C5a.
• For Use With (Application): Sandwich ELISA
• Gene Symbol: C5
• Kit Contents:
• Standard: Each vial contains 18 ng of recombinant Human Complement Component C5/C5a.
• Capture Antibody: 1 mg/mL of mouse anti-Human Complement Component C5/C5a monoclonal antibody (in PBS, pH 7.4).
• Detection Antibody: 0.2 mg/mL of mouse anti-Human Complement Component C5/C5a monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Regulatory Status: RUO
• Target: Complement Component C5/C5a

• Assay Sensitivity: 23.44-1500 pg/mL
• Conjugate: HRP
• Product Type: Antibody Pairs
• Target Species: Human
• Gene ID (Entrez): 727
• Host Species: Mouse
• Quantity: 15 Plates
• Storage Requirements: Storage is content dependent.
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.