Applied Biosystems™ TaqMan™ MGB Probe

Custom TaqMan probes are dual-labeled primers suitable for use in any real-time PCR application. They incorporate a 5' reporter dye and a 3' nonfluorescent quencher/Minor Groove Binder (MGB).

Catalog Number	Mfr. No.	Label or Dye	Quantity
A40005000	Applied Biosystems™ A40005000	ABY	6,000 pmol
A40005001	Applied Biosystems™ A40005001	ABY	20,000 pmol
A40005002	Applied Biosystems™ A40005002	ABY	50,000 pmol
A40005003	Applied Biosystems™ A40005003	JUN	6,000 pmol
A40005004	Applied Biosystems™ A40005004	JUN	20,000 pmol
A40005005	Applied Biosystems™ A40005005	JUN	50,000 pmol
A40005006	Applied Biosystems™ A40005006	Cy5	6,000 pmol
A40005007	Applied Biosystems™ A40005007	Cy5	20,000 pmol
A40005008	Applied Biosystems™ A40005008	Cy5	50,000 pmol

Description

Custom TaqMan probes are dual-labeled primers suitable for use in any real-time PCR application. They incorporate a 5' reporter dye and a 3' nonfluorescent quencher/Minor Groove Binder (MGB). Choose from FAM, VIC, ABY, JUN, Cyanine 5, TET, and NED as reporters. Probes are HPLC-purified.

Applied Biosystems TaqMan MGB (minor groove binder) probes are dual-labeled probes used for real-time PCR applications using TaqMan chemistry. TaqMan MGB Probes incorporate a 5' fluorescent reporter dye and a 3' nonfluorescent quencher (NFQ). The NFQ offers the advantage of lower background signal, which results in better precision in quantitation. Custom TaqMan MGB probes are HPLC-purified and available with FAM, VIC, ABY, JUN, Cyanine 5, TET, and NED reporter dyes.

Shorter, more specific probes

TaqMan MGB probes include a MGB moiety at the 3' end that increases the melting temperature (Tm) of the probe and stabilizes probe/target hybrids. This means that TaqMan MGB probes can be significantly shorter than traditional probes, providing better sequence discrimination and flexibility to accommodate more targets.

Nonfluorescent quencher (NFQ) maximizes sensitivity

TaqMan MGB probes incorporate an NFQ to absorb (quench) signal from the fluorescent dye label at the other end of the probe. The properties of the NFQ combined with the short length of the MGB probe result in lower background signal than with non-MGB NFQ probes. Lower background means increased sensitivity and precision in your data.

Multiplexing 

The availability of MGB probes for up to five channels allows for higher-order multiplexing, enabling  detection of more targets from the same samples. 

Guaranteed yield

The unit size you select is based on the minimum amount we will deliver, not on synthesis starting amount.

Approximate ship time

4–10 days in North America, 5-10 days in Europe

We also offer:

• Applied Biosystems custom primers and other types of TaqMan probes.
• TaqMan MGB probes and primers available as predesigned and pre-formulated TaqMan Assays.
• Commercial manufacturing products and services, including OEM and GMP oligo manufacturing.

Need a different dye or concentration or a larger lot size? The TaqMan Custom Assay and Oligo Service offers custom products that you can tailor to meet your experimental needs.

Specifications

• 3'Primer Modification: MGB-NFQ
• Format: Tube
• Green Features: Sustainable packaging
• Product Line: TaqMan™
• Purity or Quality Grade: HPLC

• Form: Liquid
• For Use With (Equipment): 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
• Internal Probe Modification: None
• Purification Method: HPLC
• For Use With (Application): Real Time PCR (qPCR)

For Research Use Only. Not for use in diagnostic procedures.