Cutaneous Lymphocyte Antigen Rat anti-Human, FITC, Clone: HECA-452, BD

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB555947	BD Biosciences 555947	100 Tests
BDB561987	BD Biosciences 561987	25 Tests

Description

The HECA-452 monoclonal antibody specifically reacts with Cutaneous Lymphocyte Associated Antigen (CLA), a carbohydrate domain shared by sialyl Lewis[x] (sLe[x]) and sialyl Lewis[a] (sLe[a]) antigens. It serves as a ligand for selectins including CD62E (E-selectin; ELAM-1). CLA is expressed on high endothelium and on lymphocytes including most T lymphocytes infiltrating cutaneous sites of inflammation. Amongst peripheral blood cells, it is expressed on monocytes and granulocytes and a subset of lymphocytes. The HECA-452 antibody is also reportedly crossreactive with the mouse CLA carbohydrate epitope that is transiently expressed by PSGL-1/CD162 on activated T cells. A number of studies suggest that CLA plays an important role in supporting leucocyte adhesive interactions and migration into extravascular tissues during inflammation.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: Cutaneous Lymphocyte Antigen
• Applications: Flow Cytometry
• Conjugate: FITC
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Isotype: IgM κ
• Purification Method: Affinity Purified
• Description: CLA; PSGL-1; CD162; P-selectin glycoprotein ligand 1; SELPL; SELPLG

• Clone: HECA-452
• Classification: Monoclonal
• Host Species: Rat
• Target Species: Human, Mouse
• Immunogen: Lymphocyte-depleted Stromal Preparation of Tonsil
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.