Description: The polyclonal antibody reacts with both mouse and rat SDF-1 alpha. It was was generated using E. coli-expressed N terminal amio acids 20-89 of mouse SDF-1 alpha as an immunogen. SDF-1 alpha (stromal cell-derived factor-1 alpha), also known as pre-B-cell growth-stimulating factor (PBSF), is a 70-amino acid CXC chemokine originally cloned from a bone marrow stromal cell line. Targeted deletion of the SDF-1 gene resulted in defects of B-cell lymphopoiesis and bone marrow myelopoiesis. SDF-1 has been shown to be chemotactic for lymphocytes. In addition, SDF-1 was recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV-1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV-1 entry. Applications Reported: This polyclonal has been reported for use in immunoblotting (WB), immunoprecipitation, and immunohistochemistry. Cross-reactivity to SDF-1 alpha of other species has not been determined. Applications Tested: To achieve best results, titrate antibody by dilution for each application as follows: Western Blot 1:1000 Immunoprecipitation 1:300-500 Immunohistochemistry 1:100-500. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.
|CXCL12 alpha (SDF-1 alpha)|
|Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot|
|PBS with 0.09% sodium azide; pH 7.2|
For Research Use Only.
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok