Cyclin D1 Antibody (CCND1/809), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2445860.1MG

Catalog No. NBP24458601

Description

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Cyclin D1 Monoclonal specifically detects Cyclin D1 in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Cyclin D1
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
• Gene Accession No.: P24385
• Gene Symbols: CCND1
• Immunogen: Recombinant human CCND1 protein
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
• Content And Storage: Store at 4C.
• Isotype: IgG2a κ

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
• Clone: CCND1/809
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, BCL1D11S287E, cyclin D1, cyclin D1 (PRAD1: parathyroid adenomatosis 1), G1/S-specific cyclin D1, G1/S-specific cyclin-D1, PRAD1 oncogene, PRAD1B-cell CLL/lymphoma 1, U21B31
• Host Species: Mouse
• Molecular Weight of Antigen: 36 kDa
• Quantity: 0.1 mg
• Research Discipline: Cancer, Cell Cycle and Replication, Core ESC Like Genes, mTOR Pathway, Stem Cell Markers, Wnt Signaling Pathway
• Gene ID (Entrez): 595
• Target Species: Human
• Form: Purified

For Research Use Only