Cyclin E1 Polyclonal Antibody, Bethyl Laboratories

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-156-0713	Bethyl Laboratories, Inc A301566AT	20 μL

Description

The recommended shelf life for this product is 1 year from date of receipt. Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Cyclin E belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.

Specifications

• Antigen: Cyclin E1
• Classification: Polyclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: TBS with 0.1% BSA and 0.09% sodium azide
• Gene Alias: CCNE
• Gene Symbols: CCNE1
• Isotype: IgG
• Purification Method: Antigen affinity chromatography
• Product Type: Antibody

• Applications: Immunohistochemistry, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: P24864
• Gene ID (Entrez): 898
• Immunogen: Between 360 and 410
• Primary or Secondary: Primary
• Content And Storage: 4° C
• Gene: CCNE1