Cytochrome c Antibody (CYCS/1010) - IHC-Prediluted, Novus Biologicals™

Catalog No.	Quantity
NBP248163	7 mL

Catalog No. NBP248163 Supplier Novus Biologicals Supplier No. NBP248163

Description

Mouse Monoclonal Antibody

Cytochrome c Monoclonal specifically detects Cytochrome c in Human, Rat samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Cytochrome c
• Applications: Immunohistochemistry, Immunohistochemistry (Paraffin)
• Classification: Monoclonal
• Clone: CYCS/1010
• Conjugate: Unconjugated
• Dilution: Immunohistochemistry, Immunohistochemistry-Paraffin
• Formulation: 1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: CYCHCS, cytochrome c, cytochrome c, somatic, THC4
• Gene Symbols: CYCS
• Host Species: Mouse
• Immunogen: Recombinant full-length human Cytochrome c protein (Uniprot: P99999)
• Molecular Weight of Antigen: 15 kDa
• Purification Method: Protein A or G purified
• Quantity: 7 mL
• Regulatory Status: RUO
• Research Discipline: Apoptosis, Cellular Markers, Cholesterol Metabolism, Core ESC Like Genes, Lipid and Metabolism, Mitochondrial Markers, Stem Cell Markers
• Primary or Secondary: Primary
• Gene ID (Entrez): 54205
• Test Specificity: Cytochrome C is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This monoclonal antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
• Target Species: Human, Rat
• Content And Storage: Store at 4C.
• Form: Purified
• Isotype: IgG2b κ