Cytochrome c Antibody (CYCS/1010), Novus Biologicals™

Catalog No.	Quantity
NBP24444601	0.1 mg
NBP24444600	0.02 mg
NBP24444602	0.2 mg

Catalog No. NBP24444601 Supplier Novus Biologicals Supplier No. NBP2444460.1MG

Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Cytochrome c Monoclonal specifically detects Cytochrome c in Human, Rat samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Cytochrome c
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Classification: Monoclonal
• Clone: CYCS/1010
• Concentration: 0.2mg/mL
• Conjugate: Unconjugated
• Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Formulation: 1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Accession No.: P99999
• Gene Alias: CYCHCS, cytochrome c, cytochrome c, somatic, THC4
• Gene Symbols: CYCS
• Host Species: Mouse
• Immunogen: Recombinant full-length human CYCS protein
• Molecular Weight of Antigen: 15 kDa
• Purification Method: Protein A or G purified
• Quantity: 0.1 mg
• Regulatory Status: RUO
• Research Discipline: Apoptosis, Cellular Markers, Cholesterol Metabolism, Core ESC Like Genes, Lipid and Metabolism, Mitochondrial Markers, Stem Cell Markers
• Primary or Secondary: Primary
• Gene ID (Entrez): 54205
• Test Specificity: Cytochrome C is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
• Target Species: Human, Rat
• Content And Storage: Store at 4C.
• Form: Purified
• Isotype: IgG2b κ

For Research Use Only