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Cytokeratin 10 Antibody (KRT10/844), Novus Biologicals™
SDP

Catalog No. NBP24470701 Shop All R&D Systems Products
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NBP24470701 0.1 mg
NBP24470700 0.02 mg
NBP24470702 0.2 mg
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Catalog No. NBP24470701 Supplier Novus Biologicals Supplier No. NBP2447070.1MG
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Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Cytokeratin 10 Monoclonal specifically detects Cytokeratin 10 in Human, Mouse samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Flow (Intracellular).

Specifications

Antigen Cytokeratin 10
Applications Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
Classification Monoclonal
Clone KRT10/844
Concentration 0.2mg/mL
Conjugate Unconjugated
Dilution Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.1 - 0.2 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
Formulation 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Accession No. P13645
Gene Alias BCIE, BIE, CK10, CK-10, cytokeratin 10, Cytokeratin-10, EHK, K10keratosis palmaris et plantaris, keratin 10, Keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris), keratin, type I cytoskeletal 10, keratin-10, KPP
Gene Symbols KRT10
Host Species Mouse
Immunogen Recombinant human KRT10 protein
Molecular Weight of Antigen 56.5 kDa
Purification Method Protein A or G purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Cytoskeleton Markers
Primary or Secondary Primary
Gene ID (Entrez) 3858
Test Specificity This MAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
Target Species Human, Mouse
Content And Storage Store at 4C.
Form Purified
Isotype IgG1 κ
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For Research Use Only

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