Cytokeratin 14 Recombinant Rabbit Monoclonal Antibody (SC65-06), Invitrogen™

Rabbit Recombinant Monoclonal Antibody

Supplier:  Thermo Scientific MA532214

Catalog No. PIMA532214

Description

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie. skin) and non-keratinizing cells (ie. mesothelial cells). At least 20 different CKs can be identified. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). Belonging to the type I subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells.

Specifications

• Antigen: Cytokeratin 14
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: TBS with 0.05% BSA, 40% Glycerol and 0.05% sodium azide, pH 7.4
• Gene Accession No.: P02533, Q61781, Q6IFV1
• Gene Symbols: KRT14
• Immunogen: Recombinant protein within mouse cytokeratin 14 aa 250-484 / 484.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunocytochemistry, Immunohistochemistry, Western Blot, Western Blot
• Clone: SC65-06
• Conjugate: Unconjugated
• Gene: KRT14
• Gene Alias: AI626930, CK14, CK-14, cytokeratin 14, cytokeratin-14, EBS3, EBS4, epidermolysis bullosa simplex, Dowling-Meara, Koebner, K14, Ka14, keratin 14, keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner), keratin 14, type I, keratin complex 1, acidic, gene 14, keratin, type I cytoskeletal 14, keratin, type I cytoskeletal 17, keratin-14, Koebner, Krt-1.14, Krt1-14, KRT14, LOC100737113, NFJ, Type I keratin Ka14
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 16664, 287701, 3861
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid