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Cytokeratin 5/8 Antibody (C-50), Novus Biologicals™
SDP

Catalog No. NBP24470401 Shop All R&D Systems Products
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NBP24470401 0.1 mg
NBP24470400 0.02 mg
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Catalog No. NBP24470401 Supplier Novus Biologicals Supplier No. NBP2447040.1MG
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Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Cytokeratin 5/8 Monoclonal specifically detects Cytokeratin 5/8 in Human, Mouse, Rat, Porcine, Bovine, Canine, Hamster, Ferret, Sheep, Chicken (Negative), Xenopus (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

Antigen Cytokeratin 5/8
Applications Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Classification Monoclonal
Clone C-50
Concentration 0.2mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Formulation 10mM PBS (pH 7.4) and 0.05% BSA with 0.05% Sodium Azide
Gene Accession No. P13647
Gene Alias cell proliferation-inducing protein 46, CK18, CK5, CYK18, DDD, K18, K1CO, K5, Keratin, Keratin 18, Keratin 5, KRT18, krt5, KRT5A, KRTB, type I cytoskeletal 18, type II cytoskeletal 5
Gene Symbols KRT5
Host Species Mouse
Immunogen Cytoskeletal preparation from HeLa cells
Purification Method Protein A or G purified
Quantity 0.1 mg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 3852
Test Specificity It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
Target Species Human, Mouse, Rat, Pig, Canine, Ferret, Hamster, Primate, Sheep, Chicken (Negative), Xenopus (Negative)
Content And Storage Store at 4C.
Form Purified
Isotype IgG1 κ
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