Cytokeratin 5/8 Antibody (SPM268), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2447030.2MG

Catalog No. NBP24470302

Description

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Cytokeratin 5/8 Monoclonal antibody specifically detects Cytokeratin 5/8 in Human, Mouse, Rat, Porcine, Canine, Ferret, Hamster, Primate, Sheep, Chicken (Negative), Xenopus (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: Cytokeratin 5/8
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Accession No.: P13647, P05787
• Gene Symbols: KRT5
• Immunogen: Cytoskeletal preparation from HeLa cells
• Quantity: 0.2 mg
• Primary or Secondary: Primary
• Test Specificity: It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: SPM268
• Conjugate: Unconjugated
• Formulation: 10mM PBS (pH 7.4) and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: cell proliferation-inducing protein 46, CK18, CK5, CYK18, DDD, K18, K1CO, K5, Keratin, Keratin 18, Keratin 5, KRT18, krt5, KRT5A, KRTB, type I cytoskeletal 18, type II cytoskeletal 5
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Gene ID (Entrez): 3852
• Target Species: Human, Mouse, Rat, Pig, Canine, Ferret, Hamster, Primate, Sheep, Chicken (Negative), Xenopus (Negative)
• Form: Purified

For Research Use Only