Cytokeratin 5/8 Antibody (SPM268), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24470300	Novus Biologicals NBP2447030.02MG	0.02 mg
NBP24470301	Novus Biologicals NBP2447030.1MG	0.1 mg
NBP24470302	Novus Biologicals NBP2447030.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Cytokeratin 5/8 Monoclonal specifically detects Cytokeratin 5/8 in Human, Mouse, Rat, Porcine, Bovine, Canine, Hamster, Ferret, Sheep, Chicken (Negative), Xenopus (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: Cytokeratin 5/8
• Concentration: 0.2mg/mL
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human, Mouse, Rat, Pig, Canine, Ferret, Hamster, Primate, Sheep, Chicken (Negative), Xenopus (Negative)
• Gene Accession No.: P13647, P05787
• Gene ID (Entrez): 3852
• Immunogen: Cytoskeletal preparation from HeLa cells
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.

• Clone: SPM268
• Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Formulation: 10mM PBS (pH 7.4) and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: cell proliferation-inducing protein 46, CK18, CK5, CYK18, DDD, K18, K1CO, K5, Keratin, Keratin 18, Keratin 5, KRT18, krt5, KRT5A, KRTB, type I cytoskeletal 18, type II cytoskeletal 5
• Gene Symbols: KRT5
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

For Research Use Only