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  6. Cytokeratin 8/18 Antibody (C-43 + DC10) - Azide and BSA Free, Novus Biologicals™

Cytokeratin 8/18 Antibody (C-43 + DC10) - Azide and BSA Free, Novus Biologicals™
SDP

Catalog No. NBP247982B Shop All R&D Systems Products
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NBP247982B 0.2 mg
NBP247982A 0.1 mg
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Catalog No. NBP247982B Supplier Novus Biologicals Supplier No. NBP2479820.2MG

Description

Mouse Monoclonal Antibody

Cytokeratin 8/18 Monoclonal antibody specifically detects Antigen in Human samples. It is validated for Flow Cytometry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blotting.

Specifications

Antigen Cytokeratin 8/18
Applications Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Classification Monoclonal
Clone C-43 + DC10
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
Formulation PBS with No Preservative
Gene Alias CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, keratin-8, KO, Type-II keratin Kb8
Gene Symbols KRT8
Host Species Mouse
Immunogen Keratin preparation from a human carcinoma (C-43); PMC-42 human breast carcinoma cells (DC10)
Purification Method Protein A or G purified
Quantity 0.2 mg
Regulatory Status RUO
Research Discipline Cancer, Cytoskeleton Markers
Primary or Secondary Primary
Gene ID (Entrez) 3856
Test Specificity Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Target Species Human
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG
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