Cytokeratin 8/18 Antibody (C-43 + DC10), DyLight 680, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP247982FR

Catalog No. NB007559

Description

Cytokeratin 8/18 Monoclonal specifically detects Cytokeratin 8/18 in Human samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Cytokeratin 8/18
• Classification: Monoclonal
• Conjugate: DyLight 680
• Formulation: 50mM Sodium Borate with 0.05% Sodium Azide
• Gene Symbols: KRT8
• Immunogen: Keratin preparation from a human carcinoma (C-43); PMC-42 human breast carcinoma cells (DC10)
• Quantity: 0.1 mL
• Research Discipline: Cancer, Cell Biology, Cellular Markers, Phospho Specific
• Test Specificity: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This monoclonal antibody cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained. Immunohistochemical staining with this monoclonal antibody is indistinguishable from that obtained with monoclonal antibody 5D3.
• Content And Storage: Store at 4°C in the dark.

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: C-43 + DC10
• Dilution: Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunofluorescence
• Gene Alias: CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, keratin-8, KO, Type-II keratin Kb8
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Isotype: IgG1 κ