Cytokeratin 8/18 Antibody (KRT8/803 + KRT18/835), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2449300.1MG

Catalog No. NBP24493001

Description

Ensure accurate, reproducible results in Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Cytokeratin 8/18 Monoclonal specifically detects Cytokeratin 8/18 in Human samples. It is validated for Simple Western, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Cytokeratin 8/18
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Western Blot 0.5 - 1.0 ug/ml, Simple Western 10 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
• Gene Accession No.: P05787, P05783
• Gene Symbols: KRT8
• Immunogen: Recombinant full-length human KRT8 protein (KRT8/803); Recombinant full-length human KRT18 protein (KRT18/835)
• Quantity: 0.1 mg
• Research Discipline: Cancer, Cytoskeleton Markers
• Gene ID (Entrez): 3856
• Target Species: Human
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: KRT8/803 + KRT18/835
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, keratin-8, KO, Type-II keratin Kb8
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immuno-histochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
• Content And Storage: Store at 4C.
• Isotype: IgG

For Research Use Only