Cytokeratin, HMW Antibody (KRTH/1076) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2476900.2MG

Catalog No. NBP247690B

Description

Cytokeratin, HMW Monoclonal antibody specifically detects Antigen in Human, Rat samples. It is validated for Enzyme Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blotting.

Specifications

• Antigen: Cytokeratin, HMW
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, ELISA, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
• Gene Alias: CK-2P, cytokeratin 2, Cytokeratin-2P, HUMCYT2A, K2P, K76, keratin 76, keratin, type II cytoskeletal 2 oral, Keratin-76, KRT2Bcytokeratin-2P, KRT2Pkeratin 2p, KRT76, Type-II keratin Kb9
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Gene ID (Entrez): 51350
• Target Species: Human, Rat
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Clone: KRTH/1076
• Conjugate: Unconjugated
• Formulation: PBS with No Preservative
• Gene Symbols: KRT76
• Immunogen: Recombinant human KRT76 protein
• Quantity: 0.2 mg
• Primary or Secondary: Primary
• Test Specificity: This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50, 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ