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Cytokeratin, LMW Antibody (AE-1), Novus Biologicals™
SDP

Catalog No. NBP229411 Shop All R&D Systems Products
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NBP229411 0.1 mg
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Catalog No. NBP229411 Supplier Novus Biologicals Supplier No. NBP229411
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Description

Mouse Monoclonal Antibody

Cytokeratin, LMW Monoclonal specifically detects Cytokeratin, LMW in Human, Mouse, Rat, Bovine, Canine, Chicken, Monkey, Rabbit, Reptile samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

Antigen Cytokeratin, LMW
Applications Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Classification Monoclonal
Clone AE-1
Concentration 0.2 mg/ml
Conjugate Unconjugated
Dilution Western Blot 0.5-1 μg/mL, Flow Cytometry 0.5-1 μg/million cells, ELISA 1-5 μg/mL for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunohistochemistry-Paraffin 0.5-1 μg/mL, Immunohistochemistry-Frozen 0.5-1 μg/mL
Gene Accession No. P04264
Gene Alias Cytokeratin LMW, cytokeratin-1B, keratin 77, keratin, type II cytoskeletal 1b, KRT77, type-II keratin Kb39
Gene Symbols KRT77
Host Species Mouse
Immunogen Human epidermal keratin (Uniprot: Q7Z794)
Purification Method Protein A or G purified
Quantity 0.1 mg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 374454
Test Specificity This monoclonal antibody recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI, 48, 46, 45, and 40kDa. monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
Target Species Human, Mouse, Rat, Bovine, Canine, Chicken, Other, Primate, Rabbit
Content And Storage Store at 4C.
Form Purified
Isotype IgG1 κ
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