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Cytoskeletal Actin Rabbit anti-Human, Mouse, Polyclonal, Bethyl Laboratories

Catalog No. 501557750
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50-155-7750 100 μL
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Catalog No. 50-155-7750 Supplier Bethyl Laboratories, Inc Supplier No. A300491A
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Description

Rabbit Polyclonal Antibody

The recommended shelf life for this product is 1 year from date of receipt. Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Based on 100% sequence identity, this antibody is predicted to react with Rat, X. tropicalis, Chicken, Sheep, Bovine, Dog, Horse, Rabbit, Guinea pig_10141, Pig, Golden hamster, Orangutan and Chimpanzee

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

Specifications

Antigen Cytoskeletal Actin
Applications Immunohistochemistry, Western Blot
Classification Polyclonal
Concentration 1 mg/ml
Conjugate Unconjugated
Formulation phosphate, tris citrate with 0.09% sodium azide; pH 7-8
Gene ACTB
Gene Accession No. P60709, P60710
Gene Alias beta actin, gamma actin, cytoskeleton actin, Atcb
Gene Symbols ACTB
Host Species Rabbit
Immunogen N-terminus
Purification Method Antigen affinity chromatography
Quantity 100 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 11461, 60
Target Species Human, Mouse
Content And Storage 4° C
Product Type Antibody
Form Liquid
Isotype IgG
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