Deoxyribonuclease I Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-199-1037	Rockland Immunochemicals 20041610100	100 μg
50-199-1038	Rockland Immunochemicals 2004161S	25 μL

Description

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-DEOXYRIBONUCLEASE I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Deoxyribonuclease A [Bovine Pancreas]. Cross reactivity against Deoxyribonuclease A from other tissues and species may occur but have not been specifically determined.

Internucleosomal DNA fragmentation following the activation of endonucleases is the common end point of apoptosis. DNase I, a Ca2+/Mg2+-dependent endonuclease ubiquitously expressed in mammalian tissues, has been implicated to mediate internucleosomal DNA degradation in human cells undergoing apoptosis. DNase I is highly polymorphic, and at least six alleles of DNase I are known. DNase II, the ubiquitously expressed acidic deoxyribonuclease, acts downstream of caspase activation and may also induce DNA digestion during apoptosis. DNase I cleaves DNA to 5'-phosphodinucleotide and 5'-phospho- oligonucleotide end-products, whereas DNase II cleaves DNA to 3'-phosphomononucleotide and 3'-phosphooligonucleotide end-products. The mechanism by which DNase II cuts DNA is similar to DNase I, which produces nicks rather than double-strand cuts. DNase II is usually present in cytoplasm of epithelial cells, but it appears concentrated in the nuclei of lens fibers. In contrast, DNase I is always concentrated in nuclei of epithelial and fiber cells.

Specifications

• Antigen: Deoxyribonuclease I
• Applications: ELISA, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Bovine
• Gene Accession No.: P00639
• Gene ID (Entrez): 282217
• Immunogen: Deoxyribonuclease A [Bovine Pancreas]
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Gene: DNASE1

• Concentration: 1 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Alias: AI788650; Deoxyribonuclease 1; Deoxyribonuclease I; Deoxyribonuclease1; Deoxyribonuclease-1; DeoxyribonucleaseI; DNASE 1; DNase I; DNase I lysosomal; DNase I, lysosomal; DNASE1; DNaseI; DNL 1; Dnl1; Dornase alfa; DRNI; FLJ38093; human urine deoxyribonuclease I
• Gene Symbols: DNASE1
• Primary or Secondary: Primary
• Product Type: Antibody