Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
Learn More
Thermo Scientific™ DH5α Competent Cells, High Efficiency PROMO
High efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors.
Supplier: Thermo Scientific™ EC0112
Description
- The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal
- RecA1 and endA1 mutations in DH5α cells improve insert stability and quality of the extracted plasmid DNA
- High transformation efficiency: >1x109 cfu/μg pUC19 DNA
- Suitable for routine and high-throughput cloning applications
- Genetic markers that allow for blue/white colony screening
- Convenient two reactions per vial packaging
- S.O.C. Outgrowth medium included
- DH5α Competent Cells are suitable for variety of applications requiring high transformation efficiency:
- Efficient DNA cloning derived from PCR, cDNA-generation reactions
- recA1 marker facilitates working with difficult to transform DNA
- Ideal for generation of cDNA libraries using plasmid-derived vectors
- Site-directed mutagenesis
- Genotype: F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1
Specifications
Chemically Competent | |
Dry Ice (-80°C) | |
E. coli | |
Cloning |
Lacks F' Episome | |
DH5α | |
Tube | |
20 Reactions |
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Spot an opportunity for improvement?
Product Content Correction