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DNA-PKcs, p Thr2609 Antibody, Novus Biologicals™
SDP

Catalog No. NB600897 Shop All R&D Systems Products
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NB600897 0.1 mg
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Catalog No. NB600897 Supplier Novus Biologicals Supplier No. NB600897
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Rabbit Polyclonal Antibody

DNA-PKcs Polyclonal specifically detects DNA-PKcs in Human samples. It is validated for Western Blot, ELISA, Immunoprecipitation.

Specifications

Antigen DNA-PKcs (p Thr2609)
Applications Western Blot, ELISA, Immunoprecipitation
Classification Polyclonal
Concentration 1 mg/ml
Conjugate Unconjugated
Dilution Western Blot 1:500-1:2000, ELISA 1:300000, Immunoprecipitation 1:10 - 1:500
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
Gene Accession No. P78527, P78527, P78527, P78527
Gene Alias DNA-dependent protein kinase, DNA-dependent protein kinase catalytic subunit-like, DNA-PK catalytic subunit, DNA-PKcs, DNPK1, EC 2.7.11.1, HYRC, HYRC1, p460
Gene Symbols PRKDC
Host Species Rabbit
Immunogen DNA-PKcs [p Thr2609] antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Thr 2609 of human DNA-PKcss. (Uniprot: P78527)
Molecular Weight of Antigen 469.08 kDa
Purification Method Affinity Purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Breast Cancer, Cancer, DNA Double Strand Break Repair, DNA Repair, Non homologous end joining, Phospho Specific, Protein Kinase
Primary or Secondary Primary
Gene ID (Entrez) 5591
Test Specificity DNA-PKcss pT2609 antibody is directed against the phosphorylated form of human DNA-PKcss at the pT2609 residue. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho-specific polyclonal antibody reacts with phosphorylated pT2609 of human DNA-PKcss. Reactivity with non-phosphorylated human DNA-PKcss is minimal. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. However, cross-reactivity is expected with mouse, rat, dog, chicken and horse DNA-PKcss based on a high degree of homology to the immunogen sequence. Cross-reactivity with DNA-PKcss homologues from other sources has not been determined.
Target Species Human, Chimpanzee
Content And Storage Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
Isotype IgG
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For Research Use Only

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