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Thermo Scientific™ DNAPac™ RP Columns

Catalog No. 03100097
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Length (Metric):
10 mm
50 mm
100 mm
150 mm
250 mm
Diameter (Metric):
2.1 mm
3 mm
21.2 mm
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Catalog No. Length (Metric) Diameter (Metric)
03-100-097 150 mm 21.2 mm
DX088919 100 mm 3 mm
DX088920 50 mm 3 mm
DX088921 10 mm 3 mm
DX088923 100 mm 2.1 mm
DX088924 50 mm 2.1 mm
DX088925 10 mm 2.1 mm
03-254-124 250 mm 2.1 mm
03-100-098 10 mm 21.2 mm
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Catalog No. 03-100-097 Supplier Thermo Scientific™ Supplier No. 0809221521232
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Description

Get high resolution LC and LC-MS separation of oligonucleotides, including double-stranded (ds) DNA/RNA fragments, using these DNAPac reversed phase columns.

Achieve superior reversed phase oligonucleotide separations using the Thermo Scientific™ DNAPac™ RP HPLC columns. The unique chemistry is designed for analysis of oligonucleotides and double-stranded (ds) DNA/RNA fragments using LC-UV or LC-MS. The column chemistry provides excellent performance under a broad range of pH, temperature, and mobile phase compositions. In addition, the wide pore size of the resin provides excellent separation of large double-stranded nucleic acids up to 10k base pairs.

DNAPac RP reversed phase (RP) column is based on spherical wide-pore 4 μm polymer resin specifically designed for separation of oligonucleotides and DNA/RNA fragments. The stationary phase is highly stable to extreme pH (0-14), and temperature (5-110°C) and fully compatible with mass spectrometry friendly organic solvents and ion-pair reagents such as triethylamine (TEA) and hexylamine (HA). The wide pore size of polymer resin enables efficient separation of small to large nucleic acids with low carry-over. The column may be operated in both HPLC and UHPLC instruments, producing high resolution at high throughput for oligonucleotides and resolves both small and very large dsDNA segments:

  • Designed for ion-pair reversed-phase (IP-RP) LC and LC/MS separations of both oligonucleotides and double-stranded nucleic acids
  • High resolution and high throughput
  • Excellent MS compatibility
  • Wide operating pH range (0-14) and high temperature stability (up to 110°C)
  • Biocompatible hardware to eliminate analyte absorption
TRUSTED_SUSTAINABILITY

Specifications

Column Format Preparative column, standard
Diameter (Metric) 21.2 mm
Flow Rate 4 to 8 mL/min
Length (Metric) 150 mm
Max. Pressure 2900 psi (200 bar)
Particle Size 4 μm
pH 0 to 14
Quantity Each
Temperature up to 110°C
Stationary Phase Proprietary
Product Line DNAPac
Packing Material 4 µm polymer Resin
Column Type Reversed Phase
Phase Reversed Phase
Material DVB (Divinylbenzene) Polymer
Type Preparative Column
Technique Reversed Phase HPLC
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