Invitrogen™ DNase I Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5109650

Catalog No. PIPA5109650

Description

Immunogen sequence: SLKIAAFNIQ TFGETKMSNA TLVSYIVQIL SRYDIALVQE VRDSHLTAVG KLLDNLNQDA PDTYHYVVSE PLGRNSYKER YLFVYRPDQV SAVDSYYYDD GCEPCGNDTF NREPAIVRFF SRFTEVREFA IVPLHAAPGD AVAEIDALYD VYLDVQEKWG LEDVMLMGDF NAGCSYVRPS QWSSIRLWTS PTFQWLIPDS ADTTATPTHC AYDRIVVAGM LLRGAVVPDS ALPFNFQAAY GLSDQLAQAI SDHYPVEVML K.

Internucleosomal DNA fragmentation following the activation of endonucleases is the common end point of apoptosis. DNase I, a Ca2+/Mg2+-dependent endonuclease ubiquitously expressed in mammalian tissues, has been implicated to mediate internucleosomal DNA degradation in human cells undergoing apoptosis. DNase I is highly polymorphic, and at least six alleles of DNase I are known. DNase II, the ubiquitously expressed acidic deoxyribonuclease, acts downstream of caspase activation and may also induce DNA digestion during apoptosis. DNase I cleaves DNA to 5'-phosphodinucleotide and 5'-phospho- oligonucleotide end-products, whereas DNase II cleaves DNA to 3'-phosphomononucleotide and 3'-phosphooligonucleotide end-products. The mechanism by which DNase II cuts DNA is similar to DNase I, which produces nicks rather than double-strand cuts. DNase II is usually present in cytoplasm of epithelial cells, but it appears concentrated in the nuclei of lens fibers. In contrast, DNase I is always concentrated in nuclei of epithelial and fiber cells.

Specifications

• Antigen: DNase I
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DNASE1
• Gene Alias: AI788650; deoxyribonuclease; Deoxyribonuclease 1; deoxyribonuclease I; Deoxyribonuclease1; Deoxyribonuclease-1; DeoxyribonucleaseI; DNASE 1; DNase I; DNase I lysosomal; DNase I, lysosomal; Dnase1; DNaseI; DNL 1; DNL1; Dornase alfa; DRNI; epithelial lens deoxyribonuclease I; FLJ38093; human urine deoxyribonuclease I; pancreatic deoxyribonuclease I
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 13419, 1773, 25633
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 0.25 mg/mL
• Formulation: PBS with 50% glycerol and 0.05% ProClin 300; pH 7.3
• Gene Accession No.: P21704, P24855, P49183
• Gene Symbols: DNASE1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-282 of human DNASE1 (NP_005214.2).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG