Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase

Thermo Scientific DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.

Catalog Number	Mfr. No.	Color	Quantity
FEREP1701	Thermo Scientific™ EP1701	Colorless	200 Units
FEREP1702	Thermo Scientific™ EP1702	Colorless	500 Units
FEREP1703	Thermo Scientific™ EP1703	Colorless	2500 Units
FEREP1704	Thermo Scientific™ EP1704	Colorless	4 x 2500 U
FEREP1711	Thermo Scientific™ EP1711	Green	200 Units
FEREP1712	Thermo Scientific™ EP1712	Green	500 Units
FEREP1713	Thermo Scientific™ EP1713	Green	2500 Units
FEREP1714	Thermo Scientific™ EP1714	Green	4 x 2500 Units

Description

Thermo Scientific DreamTaq Hot Start DNA Polymerase, available in colorless and green formats, is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.

DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step. With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases. The enzyme is supplied with an optimized 10X DreamTaq Buffer containing magnesium chloride, which eliminates the need for extensive optimization of reaction conditions. DreamTaq Hot Start DNA Polymerase is also available with pre-added density reagent and electrophoresis tracking dyes, as well as in master mix format.

The 10X DreamTaq Green Buffer also includes a density reagent and two tracking dyes for convenient direct loading of PCR products on a gel.

DreamTaq Hot Start DNA Polymerase is formulated to enhance productivity through:

• Better reaction outcomes
  • Higher yield of target amplicons from low template amounts
  • Increased reaction specificity due to reliable hot-start technology
  • Wider range of amplicon lengths with routine amplification of genomic DNA fragments up to 6 kb
• Enhanced convenience
  • Room temperature reaction setup
  • Minimized optimization of Mg²⁺ concentration and of primer annealing temperatures due to optimized reaction buffer
  • Direct loading of PCR products on a gel with DreamTaq Green Buffer

Applications

• Generation of PCR products for TA cloning
• Routine PCR
• Colony PCR
• Genotyping
• RT-PCR

Specifications

• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Concentration: 5 U/μL
• Overhang: 3'-A

• Polymerase: DreamTaq DNA Polymerase
• For Use With (Application): Hot-start PCR
• Hot Start: Built-In Hot Start
• Reaction Format: Separate Components