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  5. Dryzyme™ Restriction Enzymes 

Dryzyme™ Restriction Enzymes
SDPSCI_ED

Catalog No. S94627
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Cut Site:
A.AGCTT
G.GATCC

Description

Three most frequently used restriction enzymes for biotechnology.

  • Each enzyme catalyzes cleavage at or near the defined base sequence
  • Utilized in cloning procedures
  • Restriction endonucleases are purified
  • Each lot produced meets quality control specifications

Eco RI is isolated from E. coli RY13 carrying RI (fi+ plasmid) genes for restriction and modification as well as genes for resistance to the drugs sulfathiazole and streptomycin. The recognition specificity of Eco RI is sensitive to ionic environment.

  • 5'-GAATTC-3', breaks between GA
  • 3'-CTTAAG-5', breaks between AG
Hind III - The first type restriction endonuclease activity was isolated from Haemophilus influenzae Rd cells by H. O. Smith and associates. Subsequently, the presence of two enzymes (Hind II and Hind III) in this cell strain was established.
  • 5'-AAGCTT-3', breaks between AA
  • 3'-TTCGAA-5', breaks between AA

Bam HI is isolated from Bacillus amyloliquefaciens H cells. Under certain conditions, the enzyme will recognize variations of the palindrome sequence to include GGNNCC.

  • 5'-GGATCC-3', breaks between GG
  • 3'-CCTAGG-5', breaks between GG

One restriction enzyme unit is defined as the amount of enzyme required to digest 1.0μg of lambda phage DNA in 60 min. at 37°C in a total reaction mixture of 50μL.

Order Info

  • Products are lyophilized and contain 1500 units
  • All enzyme shipments contain the appropriate reaction buffer for optimal reactions

Specifications

Concentration 500 Units
Components Appropriate reaction Buffer
Incubator Temperature 37°C
For Use With (Application) Utilized in cloning procedures
Content And Storage -20°C
Methylation Sensitivity Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not Dam Methylation-Sensitive
Enzyme Hind III
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
Type IIS RE Yes
Quantity 1500 U
Cut Site A.AGCTT
Product Type HindIII
Research Category Traditional Cloning
Form Lyophilized
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