Invitrogen™ Dynamin 1 Polyclonal Antibody

Catalog No.	Quantity
PA1660	100 μL

Catalog No. PA1660

Supplier: Invitrogen™ PA1660

Description

Rabbit Polyclonal Antibody

PA1-660 detects dynamin I (Dyn1) from rat tissues. PA1-660 has been successfully used in Western blot and immunocytochemical procedures. By Western blot, this antibody detects an ~100 kDa protein representing Dyn1 from rat whole brain extract. PA1-660 immunizing peptide corresponds to amino acid residues 633-647 from rat Dyn1. This sequence is completely conserved between rat, mouse, and human Dyn1. PA1-660 immunizing peptide (Cat. # PEP-042) is available for use in neutralization and control experiments.

Dynamin is a GTPase required for endocytosis of clathrin coated vesicles from the membrane. Dynamin's involvement in endocytosis was first discovered during the characterization of the temperature sensitive parylytic shibire mutation from Drosophila melanogaster. Three closely related isoforms have been identified in mammals. Dynamin1 is expressed exclusively in neurons, dynamin2 is ubiquitously expressed and dynamin3 is expressed primarily in the testes. Each of these isoforms of dynamin also has several splice variants. This suggests that each isoform/splice variant may have separate intracellular targets or function in the budding of distinct vesicle types. Common domain features of the dynamin proteins include an amino acid terminal GTPase domain, a central pleckstrin homology domain and a highly basic proline rich carboxyl terminus with several Src homology 3 (SH3) domains. These SH3 domains target the dynamin to the clathrin coated pit. Dynamin is known to interact with at least two proteins, AP2 adaptor protein complex and amphiphysin.

Specifications

• Antigen: Dynamin 1
• Applications: Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene: DNM1
• Gene Accession No.: P21575, P39053, Q05193
• Gene Alias: AI838169; B-dynamin; D100; Dnm; Dnm1; Dyn1; dynamin 1; Dynamin BREDNM19; dynamin i; dynamin, brain; Dynamin1; Dynamin-1; EC 3.6.5.5; EIEE31; Ftfl; Kiaa4093; mKIAA4093; Neuronal dynamin; OTTHUMP00000215500
• Gene Symbols: DNM1
• Host Species: Rabbit
• Immunogen: Synthetic Peptide corresponding to resdiues C K(633) E K A S E T E E N G S D S F(647) of rat Dyn1.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 13429, 140694, 1759
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG