E. Coli J5 LPS Antibody, Novus Biologicals™

Sheep Polyclonal Antibody

Supplier:  Novus Biologicals NB10062551

Catalog No. NB10062551

Description

E. Coli J5 LPS Polyclonal specifically detects E. Coli J5 LPS in Bacteria samples. It is validated for ELISA.

Specifications

• Antigen: E. Coli J5 LPS
• Classification: Polyclonal
• Dilution: ELISA 1:100-1:2000
• Gene Alias: Escherichia coli
• Immunogen: Heat inactivated Escherichia coli J5 (Rc mutant; ATCC)
• Quantity: 0.5 mL
• Primary or Secondary: Primary
• Target Species: Bacteria
• Form: Antisera

• Applications: ELISA
• Conjugate: Unconjugated
• Formulation: Whole antisera with 0.09% Sodium Azide
• Host Species: Sheep
• Purification Method: Unpurified
• Regulatory Status: RUO
• Test Specificity: NB100-62551 recognizes the J5 mutant of E. coli 0:111 which lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The 55 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG

For Research Use Only