Novus Biologicals™ E-Selectin/CD62E Antibody Pair [HRP]

Description

Rabbit Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Rat E-Selectin/CD62E.

Specifications

• Assay Range: 18.8 pg/mL
• Assay Sensitivity: 18.8-1200 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Rat E-Selectin/CD62E.
• Target Species: Rat
• For Use With (Application): Sandwich ELISA
• Gene Alias: CD62 antigen-like family member E, CD62E, CD62E antigen, ELAM, ELAM-1, ELAM1E-selectin, endothelial adhesion molecule 1, Endothelial leukocyte adhesion molecule 1, ESEL, LECAM2, leukocyte endothelial cell adhesion molecule 2, Leukocyte-endothelial cell adhesion molecule 2, selectin E
• Gene ID (Entrez): 6401
• Gene Symbol: SELE
• Host Species: Rabbit
• Kit Contents:
  Detection Antibody: 0.5 mg/mL of rabbit anti-E-Selectin/CD62E monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4).
  Capture Antibody: 0.4 mg/mL of rabbit anti-Rat E-Selectin/CD62E monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 33 ng of recombinant Rat E-Selectin/CD62E.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Immunology, Lipid and Metabolism
• Storage Requirements: Storage is content dependent.
• Target: E-Selectin/CD62E
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1200 pg/mL is recommended.