Invitrogen™ EB1/EB2/EB3 Monoclonal Antibody (KT65)

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MA547713

Catalog No. PIMA547713

Description

The antibody detects a 30 kDa* protein corresponding to the molecular mass of EB1 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects EB1 by immunocytochemistry in HeLa and A431 cells. The antibody may weakly detect endogenous EB2 and EB3 in some cells and tissues.

The microtubule (MT) plus-end is a crucial site for the regulation of MT dynamics and interactions by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions with organelles and macromolecular complexes. The +TIPs include diverse groups of proteins, such as motor and nonmotor proteins, MT polymerases and depolymerases as well as various regulatory and adaptor proteins. The CLIP-associated protein (CLASP) family includes CLASP1 and CLASP2 proteins, which are expressed as long (alpha) and short (beta) isoforms. Thse +TIPs conatin an N-terminal TOG domain, multiple TOG-like domains, and a basic and serine-rich motif (SxIP). The TOG domain facilitates interaction with tubulin dimers, while the SxIP motif promotes interaciton with EB1 and MTs. A C-terminal domain is involved in interaction with CLIPs, as well as several other proteins. CLASPs are MT stabilizing fators that localize to mitotic spindles, kinetochores, and the midbody. CLASPs are important for cell division, and may regulate cell migration and neuronal growth cone motility.

Specifications

• Antigen: EB1/EB2/EB3
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.05% sodium azide
• Gene Accession No.: Q15555, Q15691, Q3B8Q0, Q5XIT1, Q61166, Q66HR2, Q6PER3, Q8R001, Q9UPY8
• Gene Symbols: Mapre1, Mapre2, Mapre3
• Immunogen: Clone KT65 was generated from a GST fusion protein containing amino acids from the C-terminal region mouse EB1. This sequence is highly conserved in human and rat EB1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Western Blot, Immunocytochemistry
• Clone: KT65
• Conjugate: Unconjugated
• Gene: Mapre1
• Gene Alias: APC; Microtubule RP/EB
• Host Species: Rat
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 100732, 10982, 114764, 13589, 212307, 22919, 22924, 298848, 679221
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid