Novus Biologicals™ ECM-1/Secretory Component P85 Antibody Pair [HRP]

Description

Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human ECM-1/Secretory Component P85.

Specifications

• Assay Range: 39.06 pg/mL
• Assay Sensitivity: 39.06-2500 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human ECM-1/Secretory Component P85.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: ECM1, extracellular matrix protein 1, Secretory Component Glycoprotein, Secretory component p85
• Gene ID (Entrez): 1893
• Gene Symbol: ECM1
• Host Species: Rabbit/Mouse
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of rabbit anti-Human ECM-1/Secretory Component P85 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 1 mg/mL of mouse anti-Human ECM-1/Secretory Component P85 monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 50 ng of recombinant Human ECM-1/Secretory Component P85.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Extracellular Matrix, Immunology
• Storage Requirements: Storage is content dependent.
• Target: ECM-1/Secretory Component P85
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.8 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.