Thermo Scientific™ EcoRI, HC (50 U/µL)

The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer.

Manufacturer:  Thermo Scientific™ ER0273

Catalog No. FERER0273

Description

Take advantage of a large collection of high quality restriction enzymes with Thermo Scientific™ conventional restriction endonucleases, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

• Superior quality – stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP

Specifications

• Quantity: HC; 25,000U
• Enzyme: EcoR I
• Sensitive to Heat Inactivation: Yes

• Methylation Sensitivity: Not dam methylation-sensitive, Not dcm methylation-sensitive, CpG methylation-sensitive
• Compatible Buffer: Unique Buffer (10x Buffer EcoRI )
• Optimal Reaction Temperature: 37° C