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Elastase Antibody (CELA3B/1218), Alexa Fluor™ 532, Novus Biologicals™
SDP

Catalog No. NP247938A32 Shop All R&D Systems Products
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Catalog No. NP247938A32 Supplier Novus Biologicals Supplier No. NBP247938AF532
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Description

Mouse Monoclonal Antibody

Elastase Monoclonal specifically detects Elastase in Human samples. It is validated for Western Blot, ELISA.

Specifications

Antigen Elastase
Applications Western Blot, Flow Cytometry, ELISA, Immunofluorescence
Classification Monoclonal
Clone CELA3B/1218
Conjugate Alexa Fluor 532
Dilution Western Blot, Flow Cytometry, ELISA, Immunofluorescence
Formulation 50 mM sodium borate with 0.05% sodium azide
Gene Alias CBPP, cholesterol-binding pancreatic protease, chymotrypsin-like elastase family member 3B, chymotrypsin-like elastase family, member 3B, E1, EC 3.4.21, EC 3.4.21.70, EL-1, ELA3B, elastase 1, elastase 3B, pancreatic, Elastase IIIB, elastase-3B, fecal elastase 1, pancreatic elastase 1, pancreatic endopeptidase E, Protease E, proteinase E
Gene Symbols CELA3B
Host Species Mouse
Immunogen Recombinant human Elastase protein fragment (around aa 82-238) (exact sequence is proprietary) (Uniprot: P08861)
Quantity 0.1 mL
Primary or Secondary Primary
Gene ID (Entrez) 23436
Test Specificity This MAb recognizes a protein of ∼17kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
Target Species Human
Content And Storage Store at 4°C in the dark.
Isotype IgG1 κ
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