Elastase Antibody (CELA3B/1218), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2448120.1MG

Catalog No. NBP24481201

Description

Ensure accurate, reproducible results in ELISA, Flow Cytometry, Immunofluorescence

Elastase Monoclonal specifically detects Elastase in Human samples. It is validated for Western Blot, ELISA.

Specifications

• Antigen: Elastase
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Dilution: Western Blot, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Accession No.: P08861
• Gene Symbols: CELA3B
• Immunogen: Recombinant human CELA3B protein
• Quantity: 0.1 mg
• Research Discipline: Cancer, Neuroscience
• Gene ID (Entrez): 23436
• Target Species: Human
• Form: Purified

• Applications: Western Blot, Flow Cytometry, SDS-Page, Immunofluorescence
• Clone: CELA3B/1218
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: CBPP, cholesterol-binding pancreatic protease, chymotrypsin-like elastase family member 3B, chymotrypsin-like elastase family, member 3B, E1, EC 3.4.21, EC 3.4.21.70, EL-1, ELA3B, elastase 1, elastase 3B, pancreatic, Elastase IIIB, elastase-3B, fecal elastase 1, pancreatic elastase 1, pancreatic endopeptidase E, Protease E, proteinase E
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This MAb recognizes a protein of ∼17kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
• Content And Storage: Store at 4C.
• Isotype: IgG κ

For Research Use Only