EMD Millipore Novagen™ Veggie BL21 (DE3) Singles™ Competent Cells

Manufacturer: EMD Millipore 712524

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Catalog No. 71-252-4

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    Quantity 22 reactions
    Storage Requirements Store at ≤-70deg.C

    BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

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