Enolase 1 Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 4 publications

Supplier:  Novus Biologicals NB10065252

Catalog No. NB10065252

Description

Enolase 1 Polyclonal specifically detects Enolase 1 in Human, Mouse, Bovine samples. It is validated for Western Blot, Simple Western, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

• Antigen: Enolase 1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Phosphate buffered saline with 0.09% Sodium Azide
• Gene Alias: 2-phospho-D-glycerate hydro-lyase, Alpha enolase, C-myc promoter-binding protein, c-myc promoter-binding protein-1, EC 4.2.1, EC 4.2.1.11, ENO1L1, Enolase 1, enolase 1, (alpha), MBP-1, MBPB1, MPB-1, MPB1c-myc promoter-binding protein 1, MYC promoter-binding protein 1, NNE, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, PPHalpha enolase like 1, tau-crystallin
• Host Species: Rabbit
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This recognizes human Non-neuronal enolase, and demonstrates less than 0.1% cross-reactivity with non-specific enolase. Non-neuronal enolase is a glycolytic enzyme composed of homodimeric alpha enolase subunits and is expressed in most human tissues. The gene coding for the 48kDa form of non-neuronal enolase also codes for a shorter 37kDa isoform, myc-binding protein 1 (MBP-1) lacking the N-terminal 93 amino acids of non-neuronal enolase.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG

• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Concentration: 5 mg/mL
• Dilution: Western Blot 1:100-1:2000, Simple Western 1:5000, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:25-1:100, Immunohistochemistry-Frozen 1:25-1:100
• Gene Accession No.: P06733
• Gene Symbols: ENO1
• Immunogen: Native human Enolase 1
• Quantity: 0.5 mL
• Research Discipline: Cancer, Core ESC Like Genes, Stem Cell Markers
• Gene ID (Entrez): 2023
• Target Species: Human, Mouse, Bovine
• Form: Purified

For Research Use Only