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  6. Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) - Azide and BSA Free, Novus Biologicals™

Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) - Azide and BSA Free, Novus Biologicals™
SDP

Catalog No. NB254452200 Shop All R&D Systems Products
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NB254452200 200 μg
NB254452100 100 μg
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Catalog No. NB254452200 Supplier Novus Biologicals Supplier No. NBP254452200UG

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Description

Mouse Monoclonal Antibody

Enolase 2/Neuron-specific Enolase Monoclonal antibody specifically detects Enolase 2/Neuron-specific Enolase in Human, Mouse, Rat samples. It is validated for Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, CyTOF-ready.

Specifications

Antigen Enolase 2/Neuron-specific Enolase
Applications Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), CyTOF
Classification Monoclonal
Clone ENO2/1375
Concentration 1 mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.5 - 1 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells, Immunocytochemistry/Immunofluorescence 1 - 2 ug/ml, Immunohistochemistry-Paraffin 0.1 - 0.2 ug/ml, CyTOF-ready
Formulation PBS with No Preservative
Gene Alias 2-phospho-D-glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, EC 4.2.1.11, Enolase 2, enolase 2 (gamma, neuronal), gamma-enolase, Neural enolase, neuron specific gamma enolase, neurone-specific enolase, Neuron-specific enolase, NSE
Gene Symbols ENO2
Host Species Mouse
Immunogen A synthetic peptide corresponding to aa416-433 of human NSE gamma.
Molecular Weight of Antigen 50 kDa
Purification Method Protein A or G purified
Quantity 200 μg
Regulatory Status RUO
Research Discipline Cancer, Cellular Markers, Neuronal Cell Markers, Neuroscience
Primary or Secondary Primary
Gene ID (Entrez) 2026
Test Specificity Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Target Species Human, Mouse, Rat
Content And Storage Store at -20 to -80C. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG
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