Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NB254452100	Novus Biologicals NBP254452100UG	100 μg
NB254452200	Novus Biologicals NBP254452200UG	200 μg

Description

Enolase 2/Neuron-specific Enolase Monoclonal specifically detects Enolase 2/Neuron-specific Enolase in Human, Mouse, Rat samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Flow (Intracellular), CyTOF-ready.

Specifications

• Antigen: Enolase 2/Neuron-specific Enolase
• Concentration: 1 mg/mL
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Cellular Markers, Neuronal Cell Markers, Neuroscience
• Formulation: PBS with No Preservative
• Gene ID (Entrez): 2026
• Immunogen: A synthetic peptide corresponding to aa416-433 of human NSE gamma.
• Primary or Secondary: Primary
• Content And Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 50 kDa

• Clone: ENO2/1375
• Dilution: Western Blot 0.5 - 1 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells, Immunocytochemistry/Immunofluorescence 1 - 2 ug/ml, Immunohistochemistry-Paraffin 0.1 - 0.2 ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat
• Gene Alias: 2-phospho-D-glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, EC 4.2.1.11, Enolase 2, enolase 2 (gamma, neuronal), gamma-enolase, Neural enolase, neuron specific gamma enolase, neurone-specific enolase, Neuron-specific enolase, NSE
• Gene Symbols: ENO2
• Isotype: IgG
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

For Research Use Only