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  6. Enolase 2/Neuron-specific Enolase Mouse anti-Human, Clone: ENO2/1462, Novus Biologicals™

Enolase 2/Neuron-specific Enolase Mouse anti-Human, Clone: ENO2/1462, Novus Biologicals™
SDP

Catalog No. NBP25960220 Shop All R&D Systems Products
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NBP259602X 100 μg
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Catalog No. NBP25960220 Supplier Novus Biologicals Supplier No. NBP25960220UG
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Description

Mouse Monoclonal Antibody

Enolase 2/Neuron-specific Enolase Monoclonal specifically detects Enolase 2/Neuron-specific Enolase in Human samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array.

Specifications

Antigen Enolase 2/Neuron-specific Enolase
Applications Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), SDS-Page
Classification Monoclonal
Clone ENO2/1462
Concentration 0.2 mg/mL
Conjugate Unconjugated
Dilution Flow Cytometry 0.5-1 ug/million cells, Immunocytochemistry/Immunofluorescence 1-2 ug/ml, Immunohistochemistry-Paraffin 0.1-0.2 ug/ml, SDS-Page, Protein Array 1:100-1:2000
Formulation 10mM PBS with 0.05% BSA with 0.05% Sodium Azide
Gene Alias 2-phospho-D-glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, EC 4.2.1.11, Enolase 2, enolase 2 (gamma, neuronal), gamma-enolase, Neural enolase, neuron specific gamma enolase, neurone-specific enolase, Neuron-specific enolase, NSE
Gene Symbols ENO2
Host Species Mouse
Immunogen A synthetic peptide of human NSE gamma (around aa416-433; exact sequence is proprietary)
Molecular Weight of Antigen 50 kDa
Purification Method Protein A or G purified
Quantity 20 μg
Regulatory Status RUO
Research Discipline Cancer, Cellular Markers, Neuronal Cell Markers, Neuroscience
Primary or Secondary Primary
Gene ID (Entrez) 2026
Test Specificity The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of about 50kDa, which is identified as gamma -enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Target Species Human
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG2b
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