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GLI-2 Antibody, Novus Biologicals™
SDP

Catalog No. NB600874 Shop All R&D Systems Products
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0.025 mg
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NB600874 0.1 mg
NB600874SS 0.025 mg
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Catalog No. NB600874 Supplier Novus Biologicals Supplier No. NB600874
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Rabbit Polyclonal Antibody has been used in 6 publications

GLI-2 Polyclonal specifically detects GLI-2 in Human, Mouse, Rat samples. It is validated for Chemotaxis, ChIP Assay, ELISA, Flow Cytometry (Intracellular Staining), Immunohistochemistry, Immunohistochemistry (Paraffin), Western Blot.

Specifications

Antigen GLI-2
Applications Chemotaxis, ChIP Assay, ELISA, Flow Cytometry (Intracellular Staining), Immunohistochemistry, Immunohistochemistry (Paraffin), Western Blot
Classification Polyclonal
Concentration 1 mg/ml
Conjugate Unconjugated
Dilution Western Blot 1:500-1:2000, ELISA 1:10000-1:30000, Immunohistochemistry 1:500-1:2000, Immunohistochemistry-Paraffin 1:10-1:500, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP) 1:10-1:500
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
Gene Accession No. P10070
Gene Alias GLI family zinc finger 2, GLI-Kruppel family member GLI2, glioma-associated oncogene family zinc finger 2, HPE9tax helper protein 1, oncogene GLI2, Tax helper protein, tax helper protein 2, tax-responsive element-2 holding protein, tax-responsive element-25-bp sequence binding protein, THP, THP1, THP2, zinc finger protein GLI2
Gene Symbols GLI2
Host Species Rabbit
Immunogen This affinity purified GLI-2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-65 of human GLI-2 (isoform a). (Uniprot: P10070)
Molecular Weight of Antigen 167.78 kDa
Purification Method Affinity Purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Cancer, Stem Cell Signaling Pathway
Primary or Secondary Primary
Gene ID (Entrez) 2736
Test Specificity NB600-874 is expected to cross-react with all 4 splice variants of Human Gli as the immunogen sequence is present in all 4. Tested by Western blotting on human lung, gives low background, but no band was detected.
Target Species Human, Mouse, Rat
Content And Storage Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
Isotype IgG
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