ER beta/NR3A2 Antibody (ERb455), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP229439

Catalog No. NBP229439

Description

ER beta/NR3A2 Monoclonal specifically detects ER beta/NR3A2 in Human, Mouse, Rat, Porcine, Equine, Monkey, Sheep samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: ER beta/NR3A2
• Classification: Monoclonal
• Concentration: 0.2 mg/ml
• Dilution: Western Blot 0.5-1 μg/mL, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunohistochemistry-Paraffin 0.5-1.0 μg/mL, Immunohistochemistry-Frozen 0.5-1.0 μg/mL
• Gene Alias: Erb, ER-beta, ESR-BETA, ESTRB, estrogen receptor 2 (ER beta), estrogen receptor beta, estrogen receptor beta 4, NR3A2ESRB, Nuclear receptor subfamily 3 group A member 2
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.
• Content And Storage: Store at 4C.
• Isotype: IgG2a κ

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: ERb455
• Conjugate: Unconjugated
• Gene Accession No.: Q92731
• Gene Symbols: ESR2
• Immunogen: C-terminus fragment of recombinant human ER beta/NR3A2 protein (Uniprot: Q92731)
• Quantity: 0.1 mg
• Research Discipline: Breast Cancer, Cancer, Cell Biology, Cell Cycle and Replication, Dendritic Cell Markers, GPCR, Neuroscience, Signal Transduction, Transcription Factors and Regulators
• Gene ID (Entrez): 2100
• Target Species: Human, Mouse, Rat, Pig, Equine, Primate, Sheep
• Form: Purified

For Research Use Only