Invitrogen™ eRF1 Monoclonal Antibody (3E5)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA549272

Catalog No. PIMA549272

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human Hela whole cell, human Jurkat whole cell, human K562 whole cell, human Raji whole cell, human HEPG2 whole cell, human HEPG2 whole cell, rat liver tissue, rat testis tissue, rat kidney tissue, rat PC-12 whole cell, mouse liver tissue, mouse testis tissue, mouse kidney tissue, mouse RAW2647 whole cell. IHC: human tonsil tissue, human breast cancer tissue, human liver cancer tissue, human lung cancer tissue, rat pancreas tissue. ICC/IF: A431 cell. Flow: Caco-2 cell, HEPA1-6 cell, RH35 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors.

Specifications

• Antigen: eRF1
• Classification: Monoclonal
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and no preservative
• Gene Accession No.: P62495, Q5U2Q7, Q8BWY3
• Gene Symbols: ETF1
• Immunogen: E.coli-derived human eRF1/ETF1 recombinant protein (Position: D9-K342).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 3E5
• Conjugate: Unconjugated
• Gene: ETF1
• Gene Alias: AI463371; D5S1995; D6Ertd109e; ERF; ERF1; ETF1; Eukaryotic peptide chain release factor subunit 1; eukaryotic release factor 1; eukaryotic translation termination factor 1; polypeptide chain release factor 1; protein Cl1; RF1; sup45 (yeast omnipotent suppressor 45) homolog-like 1; SUP45L1; TB3-1
• Host Species: Mouse
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 2107, 225363, 307503
• Content And Storage: -20°C
• Form: Lyophilized