Thermo Scientific™ Exonuclease I (20 U/μL)

Description

Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.

Exonuclease I (ExoI) degrades single-stranded DNA in a 3'≥5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.

It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.

Highlights

• Active in PCR buffers

Applications

• Primer removal from PCR mixtures:
  • prior to PCR product sequencing
  li>for one-tube "megaprimer" PCR mutagenesis
• Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
• Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus

Note

The enzyme is not suitable for removing 3'-overhangs of dsDNA.

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Specifications

• Concentration: 20 U/μL
• Enzyme: Exonuclease
• Quantity: 4,000 units
• Product Type: Exonuclease I

For Research Use Only. Not for use in diagnostic procedures.