Novus Biologicals™ Factor XIII Antibody Pair [HRP]

Description

Polyclonal/Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes an antibody specific for Human Factor XIII.

Specifications

• Assay Range: 6.25 pg/mL
• Assay Sensitivity: 6.25-400 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Human Factor XIII.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: bA525O21.1 (coagulation factor XIII, A1 polypeptide), coagulation factor XIII A chain, coagulation factor XIII, A1 polypeptide, Coagulation factor XIIIa, EC 2.3.2.13, F13Acoagulation factor XIII, A polypeptide, factor XIIIa, fibrin stabilizing factor, A subunit, fibrinoligase, FSF, A subunit, Protein-glutamine gamma-glutamyltransferase A chain, TGase, Transglutaminase A chain, transglutaminase. plasma
• Gene ID (Entrez): 2165
• Gene Symbol: F13B
• Host Species: Rabbit
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of rabbit anti-Human Factor XIIIb polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 0.5 mg/mL of rabbit anti-Human Factor XIIIb monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 21 ng of recombinant Human Factor XIIIb.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Storage Requirements: Storage is content dependent.
• Target: Factor XIII
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.