Thermo Scientific™ FastDigest DpnI

Cut at Gm6A^TC sites with Thermo Scientific™ FastDigest™ DpnI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: MalI).

Catalog Number	Mfr. No.	Quantity
FERFD1703	Thermo Scientific™ FD1703	50 μL (50 Reactions)
FERFD1704	Thermo Scientific™ FD1704	100 μL (100 Reactions)

Description

Lambda DNA digested with DpnI, 1.4% agarose, 22 cleavage sites

FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Highlights

• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5-15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Specifications

• Methylation Sensitivity: Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
• Compatible Buffer: 10X FastDigest Buffer/FastDigest Green Buffer
• Optimal Reaction Temperature: 37°C
• Product Type: Restriction Enzyme
• Product Line: FastDigest™
• Inactivation Temperature: 80°C

• Enzyme: Dpn I
• Sensitive to Heat Inactivation: Yes
• Type IIS RE: No
• Research Category: Traditional Cloning
• Overhang: Blunt

For Research Use Only. Not for use in diagnostic procedures.